Commit 77d934d6 authored by Poulet Camille's avatar Poulet Camille
Browse files

Merge branch 'develop' of gitlab-ssh.irstea.fr:SimAquaLife/GR3D into develop

parents de111f56 38575a86
......@@ -280,8 +280,8 @@
<mortalityRateInOffshore>0.4</mortalityRateInOffshore>
</species.Survive>
<!--<species.WriteEffectiveAndBiomassImportFluxes> <synchronisationMode>ASYNCHRONOUS</synchronisationMode> <exportSeason>SPRING</exportSeason>
<fileNameOutput>effectiveBiomassFluxesBeforeReproduction</fileNameOutput> </species.WriteEffectiveAndBiomassImportFluxes> -->
<!--<analysis.WriteEffectiveAndBiomassImportFluxes> <synchronisationMode>ASYNCHRONOUS</synchronisationMode> <exportSeason>SPRING</exportSeason>
<fileNameOutput>effectiveBiomassFluxesBeforeReproduction</fileNameOutput> </anaysis.WriteEffectiveAndBiomassImportFluxes> -->
<species.ReproduceAndSurviveAfterReproductionWithDiagnose>
<synchronisationMode>ASYNCHRONOUS</synchronisationMode>
......@@ -328,15 +328,15 @@
</processesEachStep>
<processesAtEnd>
<species.WriteNutrientImportFluxes>
<analysis.WriteNutrientImportFluxes>
<synchronisationMode>ASYNCHRONOUS</synchronisationMode>
<fileName>nutrientImportfFluxes</fileName>
</species.WriteNutrientImportFluxes>
</analysis.WriteNutrientImportFluxes>
<species.WriteNutrientExportFluxes>
<analysis.WriteNutrientExportFluxes>
<synchronisationMode>ASYNCHRONOUS</synchronisationMode>
<fileName>nutrientExportFluxes</fileName>
</species.WriteNutrientExportFluxes>
</analysis.WriteNutrientExportFluxes>
</processesAtEnd>
</processes>
......
......@@ -283,6 +283,19 @@
<!--<species.WriteEffectiveAndBiomassImportFluxes> <synchronisationMode>ASYNCHRONOUS</synchronisationMode> <exportSeason>SPRING</exportSeason>
<fileNameOutput>effectiveBiomassFluxesBeforeReproduction</fileNameOutput> </species.WriteEffectiveAndBiomassImportFluxes> -->
<analysis.AnalyseSpawnerFeatures>
<synchronisationMode>ASYNCHRONOUS</synchronisationMode>
<analysisSeason>SPRING</analysisSeason>
<memorySize>30</memorySize>
</analysis.AnalyseSpawnerFeatures>
<analysis.AnalyseFishDistribution>
<synchronisationMode>ASYNCHRONOUS</synchronisationMode>
<analysisSeason>SPRING</analysisSeason>
<memorySize>30</memorySize>
<minimumRecruitsForPopulatedBasin>50</minimumRecruitsForPopulatedBasin>
</analysis.AnalyseFishDistribution>
<species.ReproduceAndSurviveAfterReproductionWithDiagnose>
<synchronisationMode>ASYNCHRONOUS</synchronisationMode>
<reproductionSeason>SPRING</reproductionSeason>
......@@ -305,6 +318,7 @@
<displayFluxesOnConsole>false</displayFluxesOnConsole>
</species.ReproduceAndSurviveAfterReproductionWithDiagnose>
<species.MigrateFromRiverToInshore>
<synchronisationMode>ASYNCHRONOUS</synchronisationMode>
<migrationSeasonToReachInshore>SPRING</migrationSeasonToReachInshore>
......@@ -328,13 +342,15 @@
</processesEachStep>
<processesAtEnd>
<species.WriteBiomassFluxes>
<analysis.AnalyseLikelihoodOfPresence>
<synchronisationMode>ASYNCHRONOUS</synchronisationMode>
<exportSeason>SPRING</exportSeason>
<fileNameOutput>biomassFluxes</fileNameOutput>
</species.WriteBiomassFluxes>
<presenceFileName>data/input/northeastamerica/nea_presence.csv</presenceFileName>
<period>obs_1900_1950</period>
<minimumRecruitsForPopulatedBasin>50</minimumRecruitsForPopulatedBasin>
<epsilon>0.001</epsilon>
</analysis.AnalyseLikelihoodOfPresence>
<species.IdentifyPopulation>
<analysis.IdentifyPopulation>
<synchronisationMode>ASYNCHRONOUS</synchronisationMode>
<consoleDisplay>false</consoleDisplay>
<years>
......@@ -342,7 +358,7 @@
</years>
<fluxesSeason>SPRING</fluxesSeason>
<fileNameOutput>effectiveFluxes</fileNameOutput>
</species.IdentifyPopulation>
</analysis.IdentifyPopulation>
</processesAtEnd>
</processes>
<useCemetery>false</useCemetery>
......
......@@ -61,7 +61,7 @@ vonBertalanffyWithNextIncrement = function(L, L0, Linf, K, timeStepDuration, sig
}
else {
mu = log((Linf - L) * (1 - exp(-K * timeStepDuration))) - (sigma * sigma) / 2
increment = exp(rnorm(1, mu, sigma))
increment = exp(rnorm(length(mu), mu, sigma))
}
L = L + increment * tempEffect
return(L)
......@@ -101,7 +101,7 @@ spawnerSurvivalPreReproduction <- function(Triver,Tmin, Topt, Tmax){
#River survival
survProbOptRiver = survivePar$survProbOptRiver
spRiver = survProbOptRiver * TemperatureEffect(Triver, Tmin, Topt, Tmax)
spRiver = survProbOptRiver * temperatureEffect(Triver, Tmin, Topt, Tmax)
#StockAfterSurv = S * SpRiver
......@@ -159,7 +159,7 @@ stockRecruitementRelationship <- function(Triver,Tmin, Topt, Tmax, survivalStock
alleeEffect = 1/(1 + exp(-log(19)*(survivalStock - n/ratioS95_S50*surfaceWatershed)/(n*surfaceWatershed - n/ratioS95_S50*surfaceWatershed)))
Rj = (alphaj * SurvivalStock * alleeEffect)/(betaj + survivalStock * alleeEffect)
Rj = (alphaj * survivalStock * alleeEffect)/(betaj + survivalStock * alleeEffect)
#Rj = ((aj * S) * p)/(Bj +S * p)
......
---
title: "Calibration_Thermal_Response"
author: "Camille POULET"
date: "22/02/2021"
output: word_document
---
```{r setup, include=FALSE}
knitr::opts_chunk$set(echo = FALSE, include = TRUE,
warning = FALSE,
fig.cap = TRUE)
rm(list = ls())
```
```{r library, include=FALSE}
library(dplyr)
library(readr)
library(ggplot2)
library(gam)
library(mgcv)
library(XML)
library(cowplot)
library(gridExtra)
library(voxel)
library(officedown)
library(flextable)
library(tidymv)
#library(lmerTest)
```
```{r upload data, warning=FALSE}
source("../GR3D_Rdescription/GR3Dfunction.R")
source("../GR3D_Rdescription/GR3D_NEA_env_data.R")
source("../GR3D_Rdescription/GR3D_NEA_XML_parameters.R")
```
After reproduce, spawners either died (semelparous) or survived reproduction (primo-spawners).
#Survival pre-reproduction
It concerns only spawners during reproduction, and this phase has been shown highly dependent on water (Walburg and Nichols, 1967).
For the American shad (Alosa sapidissima), spawning occurs between 8°C to 26°C, with most of individuals reproduced between 14-21°C (Limburg et al, 2003).
Leggett and Whitney (1972) found a median temperature for spawning of 16-20°C for York, St Johns and Connecticut Rivers.
## Thermal range using GAMs
To adjust the temperature thresholds and enhance model predictive capacity at the northern edge of species distribution, parameter values for spawners survival before and after spawning were obtained using generalized additive models (GAM) (Hastie and Tibshirani 1990). Observations of presence/absence across the 1900-1950 period (pristine distribution) are predicted regarding the average temperature times series observed across the 98 watersheds composing the environment of GR3D-U.S.
Either summer or spring temperature are used as environmental variables depending the season at which each process occurs.
In GR3D, spawning occurs in river at the beginning of spring, so the spawner survival during reproduction was linked to spring temperature time-series in river.
```{r build a df for presence/absence over the 1900-1950 period }
#Join the preence/asbsence to river temperature
nea_presence_temp<- inner_join(tempInriver %>%
filter(between(year, 1900, 1950)) %>%
group_by(basin_id, basin_name) %>%
mutate(spring_avg = mean(spring_river_temperature),
summer_avg = mean(summer_river_temperature),
fall_avg = mean(fall_river_temperature),
winter_avg = mean(winter_river_temperature)),
nea_presence,
by = c("basin_id","basin_name"))
```
```{r}
rectTemperature = function(Tmin, Topt, Tmax, pct = 0.8, col ='red'){
optRange = thermalRange(pct, Tmin , Topt, Tmax)
rect = data.frame(xmin = c(-Inf, Tmin, optRange["upper"], Tmax),
xmax = c(Tmin, optRange["lower"], Tmax, Inf),
ymin = -Inf,
ymax = Inf,
alpha = c(.3,.6,.6,.3),
col = c(col, 'grey', 'grey', col))
return(rect)
}
```
```{r Observations of presence/absence according to temperature, include = TRUE, fig.cap= 'Temperature experienced by spawners during their river stage in spring'' }
#plot of temperature ranges
obs_spg <- nea_presence_temp %>%
ggplot() +
geom_point(aes(spring_river_temperature,obs_1900_1950), col = "orange", alpha = 0.3) +
labs(y = "Observed presences", x = "Spring river temperature (°C)") +
theme_bw()
#temperature range experienced by fish in river in spring in which presence or absence are recorded
nea_presence_temp%>%
group_by(obs_1900_1950) %>%
summarise(Tmin = min(spring_river_temperature), Tmax = max(spring_river_temperature), .groups = 'drop') %>%
flextable() %>%
autofit()
```
```{r Gam on presences absences according to spring river temperature, include = TRUE }
#gam with mgcv
gam_spg <- gam(obs_1900_1950 ~ s(spring_river_temperature),
data = nea_presence_temp,
family = binomial("logit"))
#Outputs
#statistics and p-values
summary(gam_spg)
#check
par(mfrow = c(2,2))
gam.check(gam_spg)
#-------- with mgcv ----------#
#predictions
#temp_spg <- seq(0, max(nea_presence_temp$spring_river_temperature), length.out = 100)
#log distribution
#y_pred_spg <- predict.gam(gam_spg, data.frame(spring_river_temperature = temp_spg))
#pred_spg <- predict_gam(gam_spg)
#natural distribution
#y_pred_natural_spg <- 1/(1 + exp(-y_pred_spg))
#-------- with tidyr ----------#
pred_spg <- predict_gam(gam_spg) %>% #predict_gam extract the predicted values from the gam buid with mgcv
mutate(natural_fit = 1/(1+exp(-fit))) %>% #tranform into natural scales
mutate(natural_fit_80_pct = natural_fit * 0.80) #80% of survival
tempRangeOpt <-pred_spg %>%
mutate(natural_fit_dec = as.numeric(sprintf("%.3f", natural_fit))) %>%
filter(natural_fit_dec >= 1)
```
```{r Functions for survival computation, include = TRUE }
S = 100 #number of spawners arriving at spawning grounds
temperature = seq(8,30,0.1) #temperature time series
#add the actual thermal range
rectTempRange = rectTemperature(Tmin = survivePar$TminSurv,
Topt = survivePar$ToptSurv,
Tmax = survivePar$TmaxSurv, col = 'blue')
#------- Compute spawners survival in river before reproduction --------#
SpawnerSurvivalPreRep <- spawnerSurvivalPreReproduction(nea_presence_temp$spring_river_temperature, survivePar$TminSurv,survivePar$ToptSurv, survivePar$TmaxSurv)
#------- Plot results from GAM with ggplot------------------------------#
#with ggplot
gam_gp_spg <- plotGAM(gam_spg, smooth.cov = "spring_river_temperature")+
geom_rug(data = nea_presence_temp,
aes_string(y = "obs_1900_1950", x = "spring_river_temperature"), alpha = 0.2)+
theme_bw()
#natural scales
natural_pred <-pred_spg %>%
ggplot(aes(spring_river_temperature))+
geom_line(aes(y = natural_fit))+
geom_line(aes(y = natural_fit_80_pct), col = 'red', alpha = 0.3)+
theme_bw()+
annotate("rect", xmin = rectTempRange$xmin, xmax = rectTempRange$xmax, ymin = rectTempRange$ymin, ymax = rectTempRange$ymax, alpha = rectTempRange$alpha, fill = rectTempRange$col)
#plot all plots in the same window with plot_grid
plot_grid(obs_spg, gam_gp_spg, natural_pred, labels=c("A","B","C"), ncol = 3, nrow = 1)
# plot(y_pred_natural_spg ~ temp_spg, lwd =2, type="l",
# xlab = "Spring river temperature (°C)",
# ylab = "Predicted Presences/Absences",
# xlim = c(0, 30))
# par(new = TRUE)
# plot(SpawnerSurvivalPreRep ~ nea_presence_temp$spring_river_temperature,
# lty=2,
# lwd=2,
# col = "red",
# xlab =" ",
# ylab = " ",
# xlim = c(0,30),
# xaxt="n",
# yaxt = "n")
```
```{r optimization, include = TRUE }
#Found Tmin and Tmax to have a survival of 80% accross these ranges
#--------------------------------------#
#--Initialisation of parameters values--#
#---------------------------------------#
par_0 = c(tempMinSurv = survivePar$TminSurv,
tempOptSurv = survivePar$ToptSurv,
tempMaxSurv = survivePar$TmaxSurv)
par_1 = c(tempMinSurv = 4.15,
tempOptSurv = survivePar$ToptSurv,
tempMaxSurv = 25.25)
par_ini = par_0[c('tempMinSurv', 'tempMaxSurv')]
par_ini_1 = par_1[c('tempMinSurv', 'tempMaxSurv')]
par_ini_2 = par_1[c(('tempMinSurv'))]
par_ini_3 = par_0[c(('tempOptSurv'))]
fixedPar = c(tempOptSurv = 17)
fixedPar_2 = c(tempOptSurv = 17, tempMaxSurv = 26)
fixedPar_3 = c(tempMinSurv = 4.15, tempMaxSurv = 25.25)
fixedPar_4 = c(tempMinSurv = 8, tempMaxSurv = 26)
#--------------------------------------#
#----------- Optimisation --------------#
#---------------------------------------#
spSurvival_df = nea_presence_temp
spSurvival_df %>%
group_by(obs_1900_1950) %>%
summarise(Tmin = min(spring_river_temperature),
Tmax = max(spring_river_temperature), .groups='drop') %>%
#filter(obs_1900_1950 == 1) %>%
objectiveFunction = function(par, data, fixedPar){
df <- data %>%
group_by(basin_id, basin_name,year) %>%
mutate(spSurvival = spawnerSurvivalPreReproduction(spring_river_temperature,
Tmin = fixedPar['tempMinSurv'],
Topt = par ['tempOptSurv'],
Tmax =fixedPar['tempMaxSurv']))
tempRange <- df %>%
group_by(obs_1900_1950) %>%
summarise(Tmin = min(spring_river_temperature),
Tmax = max(spring_river_temperature), .groups='drop') %>%
filter(obs_1900_1950 == 1)
df<-df %>%
filter(obs_1900_1950 == 1) %>%
group_by(basin_id,basin_name) %>%
mutate(spSurvival_opt = fifelse(spring_river_temperature == tempRange$Tmin, 0.80, fifelse (spring_river_temperature == tempRange$Tmax, 0.80,
spSurvival))) %>%
select(basin_id,basin_name,spring_river_temperature,spSurvival,spSurvival_opt,obs_1900_1950) %>%
arrange(spring_river_temperature,spSurvival)
#mutate(spSurvival_opt = fifelse(spring_river_temperature == tempRange$Tmin, 0.8 * spSurvival, fifelse (spring_river_temperature == tempRange$Tmax, 0.8 * spSurvival,
#spSurvival)))
SSE = df %>%
group_by(basin_id,basin_name) %>%
filter(spSurvival != spSurvival_opt) %>%
mutate(SSE = (spSurvival - spSurvival_opt)^2) %>%
ungroup() %>%
summarise(sum(SSE), .groups = 'drop') %>%
unlist(use.names = FALSE)
return(SSE)
}
#objectiveFunction(par_0,spSurvival_df)
res <- optim(par = par_1,
fn = objectiveFunction,
data = spSurvival_df)
#method = "L-BFGS-B",
#lower = c(-10,14,26),
#upper = c(10,21,30))
res_3 <- optim(par = par_ini_3,
fn = objectiveFunction,
data = spSurvival_df,
fixedPar =fixedPar_3,
#method = "L-BFGS-B",
#lower = c(8),
upper = c(21))
res_4 <- optim(par = par_ini_3,
fn = objectiveFunction,
data = spSurvival_df,
fixedPar =fixedPar_4)
#method = "L-BFGS-B",
#lower = c(8),
#--------------------------------------#
#----------- Plots curves --------------#
#---------------------------------------#
spSurvival_df <- spSurvival_df %>%
group_by(basin_id, basin_name,year) %>%
mutate(spSurvivalOpt = spawnerSurvivalPreReproduction(spring_river_temperature,
Tmin = res$par['tempMinSurv'],
Topt = res$par['tempOptSurv'],
Tmax = res$par['tempMaxSurv']),
spSurvival = spawnerSurvivalPreReproduction(spring_river_temperature,
Tmin = survivePar$TminSurv,
Topt = survivePar$ToptSurv,
Tmax = survivePar$TmaxSurv)) %>%
select(basin_id,basin_name,year,spring_river_temperature,obs_1900_1950,spSurvival, spSurvivalOpt)
spSurvival_df %>%
ggplot(aes(spring_river_temperature)) +
geom_line(aes(y= spSurvival), col = "orange", alpha = 0.3) +
geom_line(aes(y= spSurvivalOpt), col = "red", alpha = 0.3)+
theme_bw()
#------------------------
```
#Juvenile survival during the reproduction
After hatching, juveniles spend their first summer in freshwater before emigrating seaward, in the inshore basin to spend fall.
Considering that spawner reproduce in spring and a larvae survival 14dph, we used the spring riverine temperature for thermal range optimisation.
TO COMPLETE BY THERMAL RANGE
```{r optimisation with gam }
S = 100 #number of spawners arriving at spawning grounds
temperature = seq(8,30,0.1) #temperature time series
#add the actual thermal range
rectTempRangeLarv = rectTemperature(Tmin = reproducePar$TminRep,
Topt = reproducePar$ToptRep,
Tmax = reproducePar$TmaxRep, col = 'blue')
#natural scales
natural_pred_larv <-pred_spg %>%
ggplot(aes(spring_river_temperature))+
geom_line(aes(y = natural_fit))+
geom_line(aes(y = natural_fit_80_pct), col = 'red', alpha = 0.3)+
theme_bw()+
annotate("rect", xmin = rectTempRangeLarv$xmin, xmax = rectTempRangeLarv$xmax, ymin = rectTempRangeLarv$ymin, ymax = rectTempRangeLarv$ymax, alpha = rectTempRangeLarv$alpha, fill = rectTempRangeLarv$col)
#plot all plots in the same window with plot_grid
plot_grid(obs_spg, gam_gp_spg, natural_pred_larv, labels=c("A","B","C"), ncol = 3, nrow = 1)
```
```{r optimization, include = TRUE }
#Found Tmin and Tmax to have a survival of 80% accross these ranges
#Juvenile and recruits survival
RecruitsSurvival <- stockRecruitementRelationship(Triver = nea_presence_temp$spring_river_temperature,
Tmin = reproducePar$TminRep,
Topt = reproducePar$ToptRep,
Tmax = reproducePar$TmaxRep,
survivalStock = spawnerSurvivalPreReproduction(nea_presence_temp$spring_river_temperature, survivePar$TminSurv,survivePar$ToptSurv, survivePar$TmaxSurv))
#--------------------------------------#
#--Initialisation of parameters values--#
#---------------------------------------#
par_0 = c(tempMinRep = reproducePar$TminRep,
tempOptRep = reproducePar$ToptRep,
tempMaxRep = reproducePar$TmaxRep)
par_1 = c(tempMinRep = 4.15,
tempOptRep = survivePar$ToptSurv,
tempMaxRep = 25.25)
par_ini = par_0[c('tempMinSurv', 'tempMaxSurv')]
par_ini_1 = par_1[c('tempMinSurv', 'tempMaxSurv')]
par_ini_2 = par_1[c(('tempMinSurv'))]
par_ini_3 = par_0[c(('tempOptSurv'))]
fixedPar = c(tempOptSurv = 17)
fixedPar_2 = c(tempOptSurv = 17, tempMaxSurv = 26)
fixedPar_3 = c(tempMinSurv = 4.15, tempMaxSurv = 25.25)
fixedPar_4 = c(tempMinSurv = 8, tempMaxSurv = 26)
#--------------------------------------#
#----------- Optimisation --------------#
#--------------------------------------#
spSurvival_df = nea_presence_temp
spSurvival_df %>%
group_by(obs_1900_1950) %>%
summarise(Tmin = min(spring_river_temperature),
Tmax = max(spring_river_temperature), .groups='drop') %>%
#filter(obs_1900_1950 == 1) %>%
objectiveFunctionLarv = function(par, data, fixedPar){
df <- data %>%
group_by(basin_id, basin_name,year) %>%
mutate(spLarvSurvival = stockRecruitementRelationship(spring_river_temperature,
Tmin = par['tempMinRep'],
Topt = par ['tempOptRep'],
Tmax = par['tempMaxRep'],
survivalStock = spawnerSurvivalPreReproduction(spring_river_temperature, survivePar$TminSurv,survivePar$ToptSurv, survivePar$TmaxSurv)))
tempRange <- df %>%
group_by(obs_1900_1950) %>%
summarise(Tmin = min(spring_river_temperature),
Tmax = max(spring_river_temperature), .groups='drop') %>%
filter(obs_1900_1950 == 1)
df<-df %>%
filter(obs_1900_1950 == 1) %>%
group_by(basin_id,basin_name) %>%
mutate(spLarvSurvival_opt = fifelse(spring_river_temperature == tempRange$Tmin, 0.80, fifelse (spring_river_temperature == tempRange$Tmax, 0.80,
spLarvSurvival))) %>%
select(basin_id,basin_name,spring_river_temperature,spLarvSurvival,spLarvSurvival_opt,obs_1900_1950) %>%
arrange(spring_river_temperature,spLarvSurvival)
#mutate(spSurvival_opt = fifelse(spring_river_temperature == tempRange$Tmin, 0.8 * spSurvival, fifelse (spring_river_temperature == tempRange$Tmax, 0.8 * spSurvival,
#spSurvival)))
SSE = df %>%
group_by(basin_id,basin_name) %>%
filter(spLarvSurvival != spLarvSurvival_opt) %>%
mutate(SSE = (spLarvSurvival - spLarvSurvival_opt)^2) %>%
ungroup() %>%
summarise(sum(SSE), .groups = 'drop') %>%
unlist(use.names = FALSE)
return(SSE)
}
#objectiveFunction(par_0,spSurvival_df)
res <- optim(par = par_1,
fn = objectiveFunctionLarv,
data = spSurvival_df)
#method = "L-BFGS-B",
#lower = c(-10,14,26),
#upper = c(10,21,30))
res_3 <- optim(par = par_ini_3,
fn = objectiveFunction,
data = spSurvival_df,
fixedPar =fixedPar_3,
#method = "L-BFGS-B",
#lower = c(8),
upper = c(21))
res_4 <- optim(par = par_ini_3,
fn = objectiveFunction,
data = spSurvival_df,
fixedPar =fixedPar_4)
#method = "L-BFGS-B",
#lower = c(8),
#--------------------------------------#
#----------- Plots curves --------------#
#---------------------------------------#
spSurvival_df <- spSurvival_df %>%
group_by(basin_id, basin_name,year) %>%
mutate(spSurvivalOpt = spawnerSurvivalPreReproduction(spring_river_temperature,
Tmin = res$par['tempMinSurv'],
Topt = res$par['tempOptSurv'],
Tmax = res$par['tempMaxSurv']),
spSurvival = spawnerSurvivalPreReproduction(spring_river_temperature,
Tmin = survivePar$TminSurv,
Topt = survivePar$ToptSurv,
Tmax = survivePar$TmaxSurv)) %>%
select(basin_id,basin_name,year,spring_river_temperature,obs_1900_1950,spSurvival, spSurvivalOpt)
spSurvival_df %>%
ggplot(aes(spring_river_temperature)) +
geom_line(aes(y= spSurvival), col = "orange", alpha = 0.3) +
geom_line(aes(y= spSurvivalOpt), col = "red", alpha = 0.3)+
theme_bw()
#------------------------
```